Journal Guides5 min readUpdated Apr 28, 2026

Microorganisms Submission Guide

A practical Microorganisms submission guide for microbiology researchers evaluating their work against the MDPI microbiology bar.

Senior Researcher, Molecular & Cell Biology

Author context

Specializes in molecular and cell biology manuscript preparation, with experience targeting Molecular Cell, Nature Cell Biology, EMBO Journal, and eLife.

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Quick answer: This Microorganisms submission guide is for microbiology researchers evaluating their work against the MDPI microbiology bar. The journal is moderately selective (~50-55% acceptance, 20-30% desk rejection). The editorial standard requires substantive microbiology contributions.

If you're targeting Microorganisms, the main risk is weak microbiology contribution, methodological gaps, or missing microbiology framing.

From our manuscript review practice

Of submissions we've reviewed for Microorganisms, the most consistent desk-rejection trigger is weak microbiology contribution despite the higher acceptance rate.

How this page was created

This page was researched from Microorganisms' author guidelines, MDPI editorial-policy materials, Clarivate JCR data, and Manusights internal analysis of submissions.

Microorganisms Journal Metrics

Metric
Value
Impact Factor (2024 JCR)
4.1
5-Year Impact Factor
~4.5+
CiteScore
7.5
Acceptance Rate
~50-55%
Desk Rejection Rate
~20-30%
First Decision
2-4 weeks
APC (Open Access)
$2,600 (2026)
Publisher
MDPI

Source: Clarivate JCR 2024, MDPI editorial disclosures (accessed April 2026).

Microorganisms Submission Requirements and Timeline

Requirement
Details
Submission portal
MDPI submission system
Article types
Article, Review, Communication
Article length
8-15 pages
Cover letter
Required
First decision
2-4 weeks
Peer review duration
4-8 weeks

Source: Microorganisms author guidelines.

Submission snapshot

What to pressure-test
What should already be true before upload
Microbiology contribution
Substantive microbial advance
Methodological rigor
Validated experimental methods
Microbiology framing
Direct relevance to microbiology
Reproducibility
Strain identification and protocols
Cover letter
Establishes the microbiology contribution

What this page is for

Use this page when deciding:

  • whether the microbiology contribution is substantive
  • whether methodology is rigorous
  • whether microbiology framing is articulated

What should already be in the package

  • a clear microbiology contribution
  • rigorous methodology
  • microbiology framing
  • reproducibility (strain ID, protocols)
  • a cover letter establishing the contribution

Package mistakes that trigger early rejection

  • Weak microbiology contribution.
  • Methodological gaps.
  • Missing microbiology framing.
  • General biology without microbiological focus.

What makes Microorganisms a distinct target

Microorganisms is a flagship MDPI microbiology journal.

Microbiology standard: the journal differentiates from broader life-sciences venues by demanding microbiology contributions.

Methodological-rigor expectation: editors expect validated experimental methods.

The 20-30% desk rejection rate: initial editorial screen.

What a strong cover letter sounds like

The strongest Microorganisms cover letters establish:

  • the microbiology contribution
  • the methodological approach
  • the microbiology framing
  • the central finding

Diagnosing pre-submission problems

Problem
Fix
Weak contribution
Articulate microbiology advance
Methodological gaps
Strengthen experimental support
Missing microbiology framing
Articulate microbiology relevance

How Microorganisms compares against nearby alternatives

Method note: the comparison reflects published author guidelines and Manusights internal analysis. We have not personally been Microorganisms authors; the boundary is publicly documented editorial behavior. Pros and cons are based on documented editorial scope.

Factor
Microorganisms
Frontiers in Microbiology
Applied and Environmental Microbiology
mBio
Best fit (pros)
MDPI broad microbiology
Frontiers broad
ASM applied + environmental
ASM mechanistic broad
Think twice if (cons)
Topic is highly novel
Topic is highly novel
Topic is non-applied
Topic is incremental

Submit If

  • the microbiology contribution is substantive
  • methodology is rigorous
  • microbiology framing is direct
  • reproducibility is appropriate

Think Twice If

Before upload, run your manuscript through a Microorganisms microbiology check.

In our pre-submission review work with manuscripts targeting Microorganisms

In our pre-submission review work with microbiology manuscripts targeting Microorganisms, three patterns generate the most consistent desk rejections.

In our experience, roughly 35% of Microorganisms desk rejections trace to weak microbiology contribution. In our experience, roughly 25% involve methodological gaps. In our experience, roughly 20% arise from missing microbiology framing.

  • Weak microbiology contribution. Editors look for substantive advances. We observe submissions framed as marginal extensions routinely desk-rejected.
  • Methodological gaps. Editors expect validated experimental methods. We see manuscripts with thin methodology routinely returned.
  • Missing microbiology framing. Microorganisms specifically expects microbiological focus. We find papers framed as general biology without microbiology positioning routinely declined. A Microorganisms microbiology check can identify whether the package supports a submission.

Clarivate JCR 2024 bibliometric data places Microorganisms among microbiology journals.

What we look for during pre-submission diagnostics

In pre-submission diagnostic work for top microbiology journals, we consistently see four signals that distinguish strong submissions from weak ones. First, the contribution must be substantive. Second, methodology should be rigorous. Third, microbiology framing should be primary. Fourth, reproducibility should be appropriate.

How microbiology framing matters

The single most consistent feedback class we deliver in pre-submission diagnostics for Microorganisms is the general-versus-microbiology distinction. Editors expect microbiology contributions. Submissions framed as general biology without microbiology positioning routinely receive "where is the microbiology contribution?" feedback. We coach authors to lead with the microbiology question.

Common pre-submission diagnostic patterns we encounter

Beyond the rubric checks, three pre-submission diagnostic patterns recur most often in the manuscripts we review for Microorganisms. First, manuscripts where the abstract reports findings without microbiology framing are flagged. Second, manuscripts where methodology lacks strain identification are flagged. Third, manuscripts that lack engagement with Microorganisms' recent issues are flagged.

What separates strong from weak submissions at this tier

The strongest manuscripts we coach distinguish themselves on three operational behaviors. First, they confine the cover letter to one page. Second, they include a one-sentence elevator pitch. Third, they identify the specific recent Microorganisms articles that this manuscript builds on.

How editorial triage shapes submission strategy

Editorial triage at Microorganisms operates on limited time per manuscript. Editors typically scan abstract, introduction, methodology, and conclusions before deciding whether to invite reviewer engagement. We coach researchers to design abstract, introduction, and conclusions for fast assessment.

Author authority and editorial-conversation positioning

Beyond methodology and contribution, Microorganisms weights author-team authority within the microbiology subfield. Strong submissions reference Microorganisms' recent papers explicitly.

Reviewer expectations vs editorial expectations

A useful diagnostic distinction is between editor expectations and reviewer expectations. Editors triage on fit and apparent rigor; reviewers evaluate technical depth. The strongest manuscripts pass both filters.

Why specific subfield positioning matters at this tier

Beyond methodology and contribution, journals at this tier increasingly reward submissions that explicitly position the work within a specific subfield conversation rather than treating the literature as undifferentiated.

How synthesis arguments differ from comprehensive surveys

The single most consistent feedback class we deliver is the synthesis-versus-survey distinction. A comprehensive survey catalogs recent papers. A synthesis offers an organizing framework. We coach researchers to articulate their organizing argument in one sentence before drafting.

Common pre-submission diagnostic patterns we observe at this tier

Beyond the rubric checks, three pre-submission diagnostic patterns recur most often. First, manuscripts where the abstract leads with context lose force. Second, manuscripts where the methods lack quantitative rigor are flagged. Third, manuscripts that lack engagement with the journal's recent issues are at risk.

Final pre-submission checklist

Manuscripts checking these five items consistently clear the editorial screen at higher rates within the broader microbiology community: (1) clear microbiology contribution; (2) rigorous methodology; (3) microbiology framing; (4) reproducibility through strain identification and detailed protocols; (5) discussion of broader microbiology implications.

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Final operational checklist for editors and reviewers

We use a final operational checklist with researchers before submission, designed to satisfy both editor triage and reviewer-level evaluation. The package should include: a clear contribution statement in the cover letter's first paragraph that articulates the substantive advance; explicit identification of the journal's three-to-five most recent papers this manuscript builds on or differentiates from; quantitative comparison against state-of-the-art baselines with statistical significance testing where applicable; comprehensive validation appropriate to the research question, including sensitivity analyses where relevant; and a discussion section that explicitly articulates limitations, computational complexity considerations where relevant, and future research directions integrated into the conclusions rather than treated as an afterthought to the broader microbiology community.

Frequently asked questions

Submit through MDPI's submission system. The journal accepts unsolicited Articles, Reviews, and Communications on microbiology. The cover letter should establish the microbiology contribution.

Microorganisms' 2024 impact factor is around 4.1. Acceptance rate runs ~50-55% with desk-rejection around 20-30%. Median first decisions in 2-4 weeks.

Original research on microbiology: bacteriology, virology, mycology, microbial ecology, microbiome, and emerging microbiology topics.

Most reasons: weak microbiology contribution, methodological gaps, missing microbiology framing, or scope mismatch.

References

Sources

  1. Microorganisms author guidelines
  2. Microorganisms homepage
  3. MDPI editorial policies
  4. Clarivate JCR 2024: Microorganisms

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