PLOS ONE Pre-Submission Checklist: Are You Ready to Submit?

Methods and reproducibility

1. Are the methods detailed enough for another lab to reproduce the work?

This is where PLOS ONE is actually more demanding than many selective journals. Because the journal reviews for soundness specifically, the methods section gets more scrutiny than at journals where editors focus on impact. Every statistical test must be named, justified, and matched to the data type. Sample sizes must be explained. Software versions must be specified.

Ask yourself: if someone in your field read only the methods section, could they reproduce the study? If the answer is "mostly, but they would need to email me about a few details," the methods are not ready.

2. Is the sample size justified?

PLOS ONE expects either a formal power analysis or a clear explanation of the practical constraints that determined the sample size. "We used 30 participants" without justification raises reviewer questions. "We used 30 participants based on a power analysis targeting 80% power to detect a medium effect size (d=0.5) at alpha=0.05" does not.

3. Are all statistical tests appropriate for the data?

Parametric tests on non-normal data. t-tests with more than two groups. Correlation interpreted as causation. PLOS ONE reviewers check these specifically because methodological validity is the journal's core editorial standard.

Data availability

4. Is the data deposited in a public repository?

PLOS ONE requires that underlying data be publicly available. "Data available upon request" is not sufficient unless there is a specific, justified reason (human subjects restrictions, endangered species locations). The statement must include repository names, accession numbers, or DOIs.

Preferred repositories: Dryad, Figshare, GenBank, or a field-specific option. If you have not deposited the data yet, do it before submission. This is not a step you can defer.

5. Is the data availability statement specific?

A vague statement delays the process. "All data are available in the Supporting Information files" is acceptable only if the data are actually there. "Data are available from the corresponding author" without explaining why public deposit is not possible will be sent back for clarification.

Reporting standards

6. Have you completed the correct reporting checklist?

PLOS ONE requires discipline-specific reporting guidelines:

The checklist must be completed with specific page/section references, not generic pointers. Check the EQUATOR Network if unsure which guideline applies.

7. Is the clinical trial registered (if applicable)?

PLOS ONE requires prospective registration of clinical trials before participant enrollment begins. The registry name and number must appear in the abstract. Manuscripts describing unregistered trials will be returned without review.

Conclusions and claims

8. Do the conclusions stay within what the evidence supports?

PLOS ONE is especially sensitive to overclaiming. The journal does not evaluate significance, which means the editorial filter focuses even more on whether claims are proportional to the evidence. An observational study described with causal language will be flagged. A pilot study presented as definitive will be sent back.

Match the language to the design: "suggests" for associative findings, "is consistent with" for confirmatory results, "demonstrates" only when the design truly supports it.

Ethics and compliance

9. Are ethics approvals explicitly stated?

Human subjects research needs IRB approval with the institution named and the approval number stated in the methods. Animal research needs institutional animal care committee approval. Clinical trials need both ethics approval and registration. PLOS takes research integrity seriously and incomplete ethics documentation can stop a paper before review.

10. Is the conflict of interest declaration complete?

All authors must declare conflicts. "No conflicts" requires each author to have confirmed this. Undisclosed conflicts discovered after publication lead to corrections or retractions.

Formatting and logistics

11. Is the manuscript in an accepted format?

PLOS ONE accepts DOC, DOCX, and RTF. LaTeX must be submitted as PDF. Figures must be separate files (not embedded), cited in ascending order, with captions in the manuscript text. Supporting information files have a 20 MB limit each. The abstract must be under 300 words with no citations.

12. Does the manuscript read like it was prepared for PLOS ONE?

A paper rejected from a selective journal and resubmitted to PLOS ONE without rewriting often looks structurally wrong. The introduction may oversell the significance. The discussion may overinterpret. The framing may assume reviewers will evaluate impact rather than soundness. If the manuscript was written for a different journal, rebuild it for PLOS ONE's editorial standard before submitting.